Comparative genomics of two molecular targets in animal models of hypersensitivity
Keywords:
COMPUTATIONAL BIOLOGY, HYPERSENSITIVITY, INTERLEUKIN-4, ANIMALS, LABORATORY.Abstract
Background: bioinformatics is applicable to the design of drugs, the simulation of biological effects and in the inter-species comparison of molecules involved in different phenomena and diseases, such as allergies.
Objective: to compare two key molecules in allergic conditions, by means of bioinformatic tools, between man and other animal species.
Methods: interleukin 4 (IL-4) and high affinity Fc receptor of immunoglobulin E were selected as target molecules in species: man, mouse, rat and rabbit. Data from genes were retrieved from Gene database. Genomic level comparison was carried out by Ensembl, while multiple sequence alignments were done with MUSCLE tool and identity matrices were generated by Clustal2.1. UCSC Genome Browser was used for the graphical representation of sequence alignments and the occurrence of single nucleotide polymorphisms.
Results: a greater similarity was found between coding sequences from man and rabbit for IL-4. Multiple sequence alignments for both molecules showed the highest scores of similarities in sequences from rabbit and man. Mononucleotide polymorphisms predominated in non-coding regions of IL-4.
Conclusions: the results reported here support the usefulness of rabbit as a model for human diseases related to allergies, based on the similarities for both species in terms of the two molecules that are considered key in hypersensitivity phenomena.
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