Morphometry of histological sections of the normal renal yubules in a sample of adult humans
Keywords:
KIDNEY TUBULES, DISTAL, PROXIMAL.Abstract
Background: normal renal tubules have been described by morphometry, which permits to "reconstruct" in third dimension the shape and size of the studied specimen.
Objective: to characterize the magnitude of normal renal tubules in adult humans, based on the necropsies performed at "Amalia Simoni" General Teaching Hospital from Camagüey.
Methods: a cross-sectional and descriptive study was carried out on 17 necropsies of deceased persons due to violent death, in the place herein mentioned. Two fragments of the renal cortex were processed by inclusion in paraffin, with sections of 5 μm and staining of hematoxylin and eosin. Images were taken at 400 X with an optical microscope connected to a digital camera to obtain the circularity, diameter and area of the renal tubules and their components; height, fraction of the nuclear and cytoplasmic area of the epithelium, and the number of nuclei in 255 renal tubules, 85 distal (DT) and 170 proximal (PT), through the ImageJ program.
Results: PT presented a greater magnitude than DT, with a diameter of 52,69 μm and 37,96 μm respectively, with a thicker wall and with a greater epithelial height (12,41 μm), thickness or epithelial area (1 174,9 μm2 ), and cytoplasmic area fraction (1 623,1 μm2), while in the DT the nuclear area fraction (339,9 μm2) was larger, the number of nuclei per tubules with 11 units and the light was higher in the DT with a diameter of 27,6 μm and an area of 598,3 μm2 which represents 28,65 % of the tubular magnitude.
Conclusions: PT is characterized by being of greater magnitude than DT except in the dimensions of the tubular light and the number of nuclei by tubules visible under the optical microscope.Downloads
References
Junqueira LC, Carneiro J. Histología básica textos y atlas. 6ta ed. Barcelona. España: Guanabara; 2005.
Piedrahita Echeverry VM, Prada Meza MCl, Vanegas Ruiz JJ, Vélez Echeverry C, Serna Higuita LM, Serrano Gayubo AK, et al. Causas de enfermedad renal crónica en niños atendidos en el Servicio de Nefrología Pediátrica del Hospital Universitario San Vicente de Paúl, de Medellín, Colombia. IATREIA [revista en internet]. 2011 [citado 10 de enero 2017]; 24(4). Disponible en: http://www.redalyc.org/pdf /1805/180522525001.pdf.
Méndez-Durán A, Pérez-Aguilar G, Ayala-Ayala F, Aguli Ruiz-Rosas R, Jesús González-Izquierdo J, Dávila-Torres J. Panorama epidemiológico de la insuficiencia renal crónica en el segundo nivel de atención del Instituto Mexicano del Seguro Social. Diálisis y Trasplante [revista en internet]. Dic, 2014 [citado 10 de enero 2017]; 35(4). Disponible en: http://www.elsevier.es/es-revista-dialisis-trasplante-275-resumen-panorama-epidemiologico-insuficiencia-renal-cronica-90369439.
Minello LF, Ferreira Rodrígues R, Oliveira de Oliveira LB, Tavares Rheingantz MG. Estructura Microscópica del Riñón de Caimán latirostris [en línea]. Brasil: Intituto de Biología da Universidad Federal de Pelotas; 2012 [citado 10 de enero 2017]. Disponible en: http://www.morfovirtual2012.sld.cu /index.php/morfovirtual/2012/paper/viewFile/218/316
MINSAP. Dirección de Registros Médicos y Estadísticas de Salud. Anuario Estadístico de Salud 2012. Ciudad de la Habana: MINSAP; 2013.
Murlimanju BV, Madhuri Kumar B, Kumar N, Prashanth KU, Prakash Rao Ch, Guru A, et al. Morphometric Parameters of the Human Adult Kidney: An Anatomical Study. Int J Morphol [revista en internet]. 2014 [citado 10 de enero 2017]; 32(2). Disponible en: http://dx.doi.org/10.4067/S0717-9502201400020004 5.
Pérez Mejías A, Barber Fox MO, González Núñez L, Pérez Ramos A. Morfometría renal en un biomodelo de crecimiento intrauterino retardado en ratas [CD-ROM]. La Habana: Primer Congreso Virtual de Ciencias Morfológicas; 2012.
Mendoza Jorge E, Blanco Figueredo N, Jiménez Mendoza DC, Soler Mesa L, Garcés Olivé LM. Características morfoestereológicas del glomérulo renal en fetos humanos [CD-ROM]. La Habana: Primer Congreso Virtual de Ciencias Morfológicas; 2012
Marciano DK, Brakeman PR, Lee CZ, Spivak N, Eastburn DJ, Bryant DM, et al. P120 catenin is required for normal renal tubulogenesis and glomerulogenesis. Development [revista en internet]. 2011 [citado 10 de enero 2017]; 138(10). Disponible en: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3082309/.
Lemley KV, Bertram JF, Nicholas SB, White K. Estimation of Glomerular Podocyte Number: A Selection of Valid Methods. J Am Soc Nephrol [revista en internet]. 2013 [citado 10 de enero 2017]; 24(8). Disponible en: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3736716/.
Gerritsen KG, Leeuwis JW, Koeners MP, Bakker SJ, van Oeveren W, Aten J, et al. Elevated Urinary Connective Tissue Growth Factor in Diabetic Nephropathy Is Caused by Local Production and Tubular Dysfunction. J of Diabetes Research [revista en internet]. 2015 [citado 10 de enero 2017]. Disponible en: http://www.hindawi.com/journals/jdr/2015/539787/.
Carrisoza-Gaytan R, Liu Y, Flores D, Else C, Lee HG, Rhodes G, et al. Effects of biomechanical forces on signaling in the cortical collecting duct (CCD). Am J Physiol-Ren [revista en internet]. 2014 [citado 10 de enero 2017]; 307(2). Disponible en: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4152160/.
Otaki Y, Watanabe T, Takahashi H, Kadowaki S, Narumi T, Honda Y, et al. Association of plasma thioredoxin-1 with renal tubular damage and cardiac prognosis in patients with chronic heart failure. J Cardiol [revista en internet]. 2014 [citado 10 de enero 2017]; 64(5). Disponible en: http://www.ncbi. nlm.nih.gov/pubmed/24685687.
U.S. Renal Data System. USRDS 2013 Annual Data Report: Atlas of End-Stage Renal Disease in the United States. Bethesda, Md, USA: National Institutes of Health, National Institute of Diabetes and Digestive and Kidney Diseases; 2013.
Horvatic I, Ljubanovic DG, Bulimbasic S, Knotek M, Prkacin I, Tisljar M, et al. Prognostic significance of glomerular and tubulointerstitial morphometry in idiopathic membranous nephropathy. Pathol Res Pract [revista en internet]. 2012 [citado 10 de enero 2017]; 208(11). Disponible en: http://www.ncbi.nlm.nih. gov/pubmed/22995635.
Downloads
Published
How to Cite
Issue
Section
License
This journal provides free and immediate access to its content under the principle that making research freely available to the public supports a greater exchange of global knowledge. This means that the authors transfer the copyright to the journal, so that copies and distribution of the contents can be made by any means, as long as the authors' acknowledgment is maintained. These terms are a reflection that the journal assumes copyright under Creative Commons licenses, specifically under a Creative Commons Attribution 4.0 International license.
Registration and submission of articles to the journal is free. The processing, including review, editing and publication, is completely free of charge.